RESUMO
A dose-dependent and significant (P<0.01) antiproliferative effect of Goldfussia psilostachys ethanolic extract was observed on K562 cells. The IC(50) is ca. 0.5 microg/ml. The extract markedly increases the proportion of cell in G2/M phases and decreases the population of cells in G0/G1 phases. Moreover, the antimitotic effect is correlated with polymerization of microtubule assembly. These results indicate that G. psilostachys ethanolic extract inhibits the proliferation of K562 cells and disrupts the normal dynamic of microtubules during mitosis.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Plantas Medicinais , Animais , Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/uso terapêutico , Divisão Celular/efeitos dos fármacos , Citometria de Fluxo , Humanos , Concentração Inibidora 50 , Células K562/efeitos dos fármacos , Microtúbulos/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , CoelhosRESUMO
Two new phenanthroquinolizidine alkaloids, boehmeriasins A and B, were isolated from the aqueous ethanolic extract of Boehmeria siamensis Craib (Urticaceae) by bioassay-guided fractionation. Their structures were elucidated on the basis of spectral evidence. Boehmeriasin A possesses cytotoxic activity against 12 cell lines from 6 panels of cancer including lung cancer, colon cancer, breast cancer, prostate cancer, kidney cancer and leukemia with GI (50) between 0.2 and 100 ng/mL, whereas boehmeriasin B showed lower activity.
